RE: Calculation of C classes in endoreduplicating nuclei
kukuruga%kasle1.dnet@rocdec.roc.wayne.edu
Thu, 12 Oct 1995 11:41:15 -0400
I've done extensive work on megakaryocyte ploidy, which will give, I suspect,
similar results to your corny project. A question: is ther some reason you
cannnot use straight histogram analysis techniques (set markers, integrate
the area under the curve, ratio to the total, etc.)?
If you're looking for more...have you tried MODFIT? You can pretty much set
the fitting algorithm to do anything you want.
Also, Bob Murphy has developed several fitting algorithms in his COTFIT
program, if you have access to Consort40 on a MicroVAX (Bob, are you there?).
There seems to be lots of options, unless I'm missing something in your
question.
P.S. To the folks who "cut" and "weigh"...two questions: 1) what's the c.v.
on your scissors, and 2) have you heard...they've discovered "Fire"!!!
P.P.S. I apologise for that...I couldn't resist.
CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web
http://www.cyto.purdue.edu
, EMAIL
cdrom3@flowcyt.cyto.purdue.edu
