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We detect MPO using anti-MPO-FITC from DAKO, Carpenteria, CA with the
fixation/permeabilization reagent from Pinnacle Biosystems, Burlingame,
CA. We use it on blood and bone marrow and the antibody and reagent
seems to work fine.
We use a number of other antibodies from DAKO with this
fixation/permeabilization reagent and they
work fine (anti-CD3, anti-kappa, anti-lambda, anti-PCNA, etc.)
We however almost always start with ficolled cells from blood or bone
marrow. Some whole blood studies are done after rbc lysis.
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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web