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You might try autofluorescence compensation. Thjat technique has worked
well in our Facility on cultured cells with high autofluorescence. A
reference is enclosed.
-Marty Bigos
Stanford Shared FACS Facility
Alberti S; Bucci C; Fornaro M; Robotti A; Stella M.
Immunofluorescence analysis in flow cytometry: better selection of
antibody-labeled cells after fluorescence overcompensation in the red
channel.
Journal of Histochemistry and Cytochemistry, 1991 May, 39(5):701-6.
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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web