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Anyone have any words of wisdom on optimizing conditions for the
sorting of cell surface stained drosophila cells? By the time we see the stained
cells a large percentage are "dead". Anybody have any tricks for keeping them
a little more happy during preparation (currently at room temp) and any
subsequent sorting?
Thanks,
Joe
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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web