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1. We are doing PRA's by ELISA and the results compare to flow. In the past we
have occasionally done flow PRA's with a pool of 10+ fresh cells.
2. a. LR & cadaver = yes
b. CDC = yes
c. go with "FLOW"
d. Ficolled cells or flushed lymph node cells, both T & B cells.
Linda Buckert
Loma Linda Immunology Center
Loma Linda, CA 92354
email: Linda_Buckert@ccmail.llu.edu
______________________________ Reply Separator _________________________________
Subject: flow crossmatch
Author: Joanne.Luider@CRHA-Health.Ab.Ca at InternetMail
Date: 8/18/95 2:52 PM
We need advice/information on what's happening out in the world as far as
using flow cytometry crossmatch methods vs the cytotoxicity methods (CDC).
Some of our questions are:
1. Do you screen and/or identify PRA by flow?
-do you still use/report CDC results as well?
-what do you do when the flow result is positive and CDC is negative?
_do you use pooled random donor cells to screen? Fresh/frozen?
_advantages/disadvantages?
2. Do you do flow crossmatching prospectively for renal(or other organs)
tranplants for living related or cadaver donors?
_do you still use/report CDC results as well?
-what results are used if the results differ?
-what methodology is used for flow crossmatching? ficolled cells/whole
blood lysis? T and B?
Thanks in advance everyone!
Joanne Luider
Foothills Hospital
Calgary, Alberta
phone (403)670-4765
fax (403)270-4135
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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web