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Our question has to do with doublet correction. When we observe the
raw data in (dual parameter) Peak versus Integral fluorescence scatter plots,
we sometimes observe a fair percentage of so-called doublets, i.e., cells that
have 4N amounts of integral fluorescence with 2N amounts of "peak"
fluorescence.
Our question is this: what are the pros and cons of excluding these cells
from our analysis using a gating approach versus doublet correction using
software routines? I know that this subject has been approached in other
forums, but I'd like to hear current thoughts on the matter.
Phil Marder
Lilly Research Labs
From: MARDER PHILIP (MCVAX0::MARDER)
To: cyto-inbox
cc: MARDER PHILIP (MCVAX0::MARDER)
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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web