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Jeff Edberg
Cornell University Medical Center
edbergj@hss.edu
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From: owner-cyto-sendout
To: Cytometry Mailing List
Subject: surface staining macrophages
Date: Monday, July 24, 1995 8:49PM
Hello. I'd like to look at expression of a surface protein on mouse
macrophage-
like cell lines by flow cytometry. I'm worried about decorating them with
our
mouse mAb, which could be bound by FcR's on these cells. Two options I
thought
of were 1) absorb mouse myeloma proteins to the FcR's, and FITC-label by
mAb;
2) cleave the mAb into Fab or F(ab')2 fragments which could be
FITC-conjugated.
What have other people done in these circumstances? What works and what
doesn't?
Thanks in advance,
Brett Lindenbach
Program in Immunology
Washington University - St Louis
brett@borcim.wustl.edu
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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web