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> We have been trying to determine percentage of apoptotic cells using PI
> staining and analysis of DNA histograms with Modfit LT. The program works
> very nicely, but occasionally we get a histogram in which a substantial number
> of cells appearing in the G0/G1 and the S peaks are considered by Modfit as
> apoptotic cells. Verity does not provide any "explanation" or suggestions
> regarding how to get around that. Did anyone out there encounter this same
> problem and how was it resolved? Thanx for any info.
>
> Edward F. Srour, Ph.D.
>
>
Could you post a little more info on this? I have not managed to successfully
identify apoptosis on a DNA histogram on a Facscan. The samples are FNAs of
xenografted human breast tumour cell lines.
Eric Miller, ICRF Medical Oncology Unit, Edinburgh UK
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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
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