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My self and Dr. Gary Michels have been investigating growth factor
receptors on osteosarcoma cells using flow cytometry. We have been using
a conventional monoclonal mouse-anti receptor antibody and FITC goat-anti
mouse secondary antibody. Since the receptor density per cell is less
than five hundred, there is only a small shift in fluorescence between the
positive and negative control samples.
I have heard there are methods using a triple antibody biotin avidin
combination, but that is all. Can anyone help?
Steve Vokal
Orthopedic Research
Creighton University
Omaha, Nebraska
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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web