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surfs up, gotta'go.
cp
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Brett Lindenbach wrote:
>Hello. I'd like to look at expression of a surface protein on mouse
>macrophage-
>like cell lines by flow cytometry. I'm worried about decorating them with our
>mouse mAb, which could be bound by FcR's on these cells. Two options I thought
>of were 1) absorb mouse myeloma proteins to the FcR's, and FITC-label by mAb;
>2) cleave the mAb into Fab or F(ab')2 fragments which could be
>FITC-conjugated.
>What have other people done in these circumstances? What works and what
doesn't?
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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web