Re: surface staining macrophages
S.P. Monard (spm1007@cus.cam.ac.uk)
Wed, 26 Jul 1995 09:44:13 +0100 (BST)
Hi
When staining human macrophages we would pre-incubate the cells with 2
percent human serum for 15 mins at room temp before the primary antibody
step and keep 2 percent serum in all the wash buffers thoughout staining,
this worked very well and is much less trouble than digesting and
conjugating antibodies. A common problem when staining mouse cells is
cross reactivity between mouse and rat Igs, make sure your anti-rat
secondary antibody is absorbed against mouse Igs (I assume your primary
ABs are rat).
Best of luck
Simon Monard
Cambridge
UK
CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web
http://www.cyto.purdue.edu
, EMAIL
cdrom3@flowcyt.cyto.purdue.edu
