 |
 |
 |
 |
 |
> Hi, if I remember correctly, Amershan at Illinois carries BU-1 clone anti-BrDU
> antibody.
>
>
> K. Lin
>
>
To throw a complete curve into this thread, I remember trying this Bu-1 a
long time ago for the same reason as aready given, to combine it with
surface markers. I eventually gave up after failing to get a good
separation of positive/negatives and getting a terrible DNA histogram as
well. I heard as well that a pure Bu-1 clone will not work, the reason
for denaturation was apparently a DNAase contaminant in the original
sample. I get good results on clinical and research samples using the
traditional denaturation, and I'm afraid I still cannot put a surface
marker into the assay!
Eric, Edinburgh UK
|
|
|
|
|
|
CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web