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Ray
On Fri, 30 Jun 1995, Peter D Briggs wrote:
>
> Hi, just a small question that I hope someone can answer:
>
> I'm doing research on polyploidy in several plant species and
> have been using flow to determine ploidy level. In order to quantify and
> compare, I have been running CRBC's as an external standard. After every
> five samples run, I run the CRBC's and adjust the voltage to get the CRBC
> peak at approximately channel 100 on the P-MT3 screen. My question is,
> what sort of a relationship is there between voltage and channel
> shifting? If you adjust the voltage to bring the CRBC peak up 10 channels,
> is it a linear relationship with all channels being shifted 10 as well?
> Or is there some non-linear relationship?
> Some of our samples have been giving us consistently weird
> results, and if a non-linear shift is possible, that would explain what
> we're seeing.
> Thanks in advance for your input...
>
> Peter Briggs
> pbriggs@uoguelph.ca
>
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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
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If you have any comments please direct them to
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