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Fluorescein has a pKa value of 6.4, and excitation / emission maxima at 492
/ 516 nm respectively. At pH 6.2 its fluorescence will not be dramatically
reduced vs. fluorescence at pH 7.4.
Molecular Probes, Inc. recently developed a series of fluorescein analogs
with lower pKa values and increased photostability.
These will become commercially available in the fall of this year.
Please inquire at :
Molecular Probes, Inc.
P.O. Box 22010
Eugene OR 97402
Tel : +1 - 503 - 465 - 8300 (request Technical Assistance Department)
In Europe, Tel : +31 - 71 - 233 431
Martin Poot
martin@probes.mhs.compuserve.com
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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web