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>Response to the desmosome clump problem -
>
>I support the response which warns against filtering, since it may very
>well be true the clumps may represent an important subpopulation. Filtering
>has its place, but only after all else fails.
In M. A. Hirsch, H. Lipner and R. C. Leif; "The Dissociation and Separation
of Bovine Adenohypophysial Cells". Cell Biophysics 1, pp. 93-115 (1979), we
employed a simple, continuous filtering apparatus to remove the cells from
the large clumps. This minimized the exposure of single cells and small
aggregates to the enzymes. Of greater significance, we reported our yield
in terms of DNA.
Robert C. Leif, Ph.D., PMIAC,
Vice President & Research Director
Newport Instruments
5648 Toyon Road
San Diego, CA 92115
Tel. & Fax (619) 582-0437
E-Mail rleif@crash.cts.com
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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
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