monocellular suspension
Deborah Berglund (umbbd@gemini.oscs.montana.edu)
Tue, 20 Jun 1995 12:35:47 -0600 (MDT)
In response to Mariangela's question of how to get a monocellular
suspension, it helps to have the cells in Ca and Mg free medium, centrifuge as little as
possible, and pull the cells through a syringe needle (18g). If that
doesn't work, which it usually doesn't well enough for flow, I use a
70-100 micron nylon mesh, filtering immediately prior to analysis. This
works even for the stickiest cell lines for me.
Deb Berglund
It is supposed to snow tonight!!!
CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web
http://www.cyto.purdue.edu
, EMAIL
cdrom3@flowcyt.cyto.purdue.edu
