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I have done FACS on very small things... but less than 0.2 microns!
Wow. In any case, we found that using log side scatter was a
considerably better trigger parameter than FS. In Bob Murphy's lab, we
used to centrifuge our sheath fluid at 20,000 x g to remove
particulates. This reduced the "background" event rate considerably.
Here at Stanford, I did FACS on myxococcus xanthus (an enormous 1 x 6
microns), using log side scatter--we did not have to treat the sheath
fluid specially. In both cases, the forward scatter signal was
basically meaningless. In both cases, we could easily detect
fluorescence (either fluorescein or rhodamine).
mr
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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web