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Do you find you have flourescent signal shifts doing the above, vs doing all
surface staining (3-color)? If so, do you have any idea why it happens? If
not, could you share with me your protocol for intracellular staining?
Many thanks,
Janine Jason
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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
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