Help for the TUNEL method for apoptosis
Immunobiology Research Institute, Siena (nuti@iris02.biocine.it)
Thu, 8 Jun 1995 12:53:37 +0200
Based on our experience and suggestions to set up the tecnique,the key points
for the best cell recovery during TUNEL method are:
1) Use PBS-0.1% BSA for washing the cells in every step.
2) After 70% ethanol fixation, spin down the cells at 560xg for 6 minutes at R.T.
3) In all the washing steps spin the cells at 390xg for 5 minutes at R.T.
4) After washing, remove the supernatant very carefully. Do not use the vacuum!
Following these suggestions you should be able to recover at least 50%
af your starting cells.
GOOD LUCK!!!!!
Sandra & Oretta
CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web
http://www.cyto.purdue.edu
, EMAIL
cdrom3@flowcyt.cyto.purdue.edu
