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In general do antibodies quench the fluorescence of fluorescein; sometimes
only partially. This may relate to accessability of the fluorochrome to the
antibody, for in solution do the antibodies quench the fluorescein fully if
applied at saturating conditions.
martin@probes.mhs.compuserve.com
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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web