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Yes there are several differences the main one being that annexin V is a
[Ca++] dependent binding peptide with very high affinity, removal of
calcium from the buffer causes annexin V to fall off the cell rapidly
allowing restaining after sorting. (annexin V can be fixed to the cell at
high [Ca++] if you don't want it to fall off yet wish to use a low
[Ca++] buffer after staining. I don't know about the availability of Ab but
annexin V is available conjugated to fluorescein from BRAND Applications
BV, and requests for information and supply can be made by fax +31 4499
2165 (Netherlands)
Ray
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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web