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I would like to ask some questions about Ca2+ measurement.
First, we do not have UV excitation, so we cannot use Indo-1. We try to use
Fluo-3. In our studies, the cells were incubated in a temp- and pH
controlled chamber and we hope to measure the Ca2+ up to 90 mins, i.e. to
take the cells into the flow every 5 minutes for measurement. We wonder
the dye is suitable for such long time incubation at 37C. Is there anyone
who has such experience?
Second, is there anyone who combine Fura-red and Fluo-3 for ratio
measurement? we have the problem in generating a calibration curve. Can
someone tell me how to make this calibration experiment (e.g. incubation
time, temp., concentration, etc.)
Raymond, CHANG
Institute for Surgical Research,
Klinikum Grosshadern,
University of Munich,
Germany.
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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web