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Ray
On Thu, 25 May 1995, STEVEN DAVID GORE wrote:
>
> We have been trying to adapt the TUNEL assay to identify apoptosis among
> normal human CD34+ cells, double labeled with PE-labeled CD34 antibody.
> While this procedure works well for CD34-expressing cell lines, such as
> TF-1 or KG1a (the CD34 labeling performed before formaldehyde fixation,
> then followed by MeOH or EtOH etc), among isolated bone marrow CD34+
> cells, CD34 appears undetectable after the alcohol second fixation step
> (either alcohol). Does anyone have any experience or suggestions?
>
>
>
> Steve Gore
> Johns Hopkins Oncology Center
> Oncology 2-109
> 600 N. Wolfe St.
> Balto, MD 21287-8963
> sdgore@welchlink.welch.jhu.edu
> 410-955-8781
> FAX 410-614-1005
>
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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
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If you have any comments please direct them to
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Phone: (765)-494-0757;
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