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Two questions:
1. Is there any problem with my laser setup?
2. What are the optimal dichroic mirrors and filters for
Hoechst 33342? The Epics 753 did not come with a standard setup
for Hoechst. I have tried all the combinations that I can think
of from the Epics standard filter set, but get only scattered
light coming through the PMT from the blue laser. The only
laser blocking filter in the standard set for the 753 blocks all
emission from 430-520, which is exactly where Hoechst emits.
I'd greatly appreciate any help.
Tony Bakke
e-mail: bakkea@ccmail.ohsu.edu
FAX: (503) 494-5799
phone (503) 494-4687
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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web