Apoptosis in CD34+ cells
STEVEN DAVID GORE (sdgore@welchlink.welch.jhu.edu)
Thu, 25 May 1995 20:43:34 -0400 (EDT)
We have been trying to adapt the TUNEL assay to identify apoptosis among
normal human CD34+ cells, double labeled with PE-labeled CD34 antibody.
While this procedure works well for CD34-expressing cell lines, such as
TF-1 or KG1a (the CD34 labeling performed before formaldehyde fixation,
then followed by MeOH or EtOH etc), among isolated bone marrow CD34+
cells, CD34 appears undetectable after the alcohol second fixation step
(either alcohol). Does anyone have any experience or suggestions?
Steve Gore
Johns Hopkins Oncology Center
Oncology 2-109
600 N. Wolfe St.
Balto, MD 21287-8963
sdgore@welchlink.welch.jhu.edu
410-955-8781
FAX 410-614-1005
CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web
http://www.cyto.purdue.edu
, EMAIL
cdrom3@flowcyt.cyto.purdue.edu
