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> Do
> people believe there is evidence to discriminate CD33+CD34+ cells from
> CD33-CD34+ cells? /Michael Kornstein, MD, Medical College of Virginia,
There are clearly CD34+CD33- cells. These include the CD34+CD10+CD19+ B
lineage precursors, the rare CD34+ CD7+ putative T cell precursors, as
well as less differentiated CD34+ cells. However, since CD33 staining is
not bright among CD34+ cells, one certainly does not see clearcut
"positive" and "negative" populations. If your interest is in picking
out "lineage negative" CD34+ cells, you might do better looking at CD38
negative or low cells (Terstappen et al Blood 77:1218-1227, 1991, among
other publications)
Steve Gore
Johns HOpkins Oncology Center
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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web