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We type acute leukaemia patients, at presentation, for a number of
markers; CD7,CD10,CD19,CD13,CD33 and CD34. Currently we stain for
single markers only with these antibodies, on ficoll separated
cells.
We would like to perform dual and tri colour analysis on whole
bone marrow. We wish to identify a unique cell marker combination on
the malignant cell population for each patient. Once established we
would like to use this unique set of markers to look for residual
disease or relapse cells in marrow.
Any ideas on a good protocol?
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| Alan Bishop |
| Flow Cytometry Laboratory |
| Hanson Centre for Cancer Research |
| Institute of Medical and Veterinary Science |
| PO Box 14 Rundle Mall |
| Adelaide SA 5000 |
| AUSTRALIA |
| |
| Voice : 61-8-228 7258 |
| Fax : 61-8-232 4092 |
| Email : bishop@immuno.imvs.gov.sa.au |
| |
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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web