Sorting Question
Vincent Falco (vincent.falco@es.nemc.org)
Thu, 19 Jun 97 15:20:18 -24000
My problem is this:During some sorts I get ,what I will call a spurious extra
drop. To explain more,there are times I sort to polycarb filters.When all is
well I get a single spot which contains the desired cell population.When all
is supposed not well I get my desired single spot with cells and just about a
quarter of inch from the desired spot I observe a spurious spot,which does
not contain the desired cells.I maintain the nozzle on a strict PM cleaning
schedule and watch for clogs etc.during times of sorting.The drive frequency
remains constant during a sort and is generally consistent day to day.The
amplitude level requires adjustment during a sort run.Should I be suspect
that the amplitude level is giving the problem? Can anyone suggest other
potential problem areas.Can anyone give rules/guidelines they follow when
establishing drop drive frequency and amplitude level?Thanks.
CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web
http://www.cyto.purdue.edu
, EMAIL
cdrom3@flowcyt.cyto.purdue.edu
