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> > Looking for a good permeableization reagent for TdT staining. Any
good > one's out there? Am planning to try Triton-X and CalTag's. Any
other > good procedures that could be used for bone marrows with 2 or 3
color > staining?
We published several years ago on formaldehyde/tx-100 for two color
staining (surface label before fixation). I have found that the
commercial products such as bd's don't always give adequate
permeabilization, although Mike Borowitz in heme/path here thinks its
terrific.
Steve Gore Johns Hopkins Oncology Center
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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web