Re: Texas Red Laser Excitation
Howard Shapiro (hms@shapirolab.com)
Fri, 3 May 1996 10:32:35 -0400 (EDT)
Historically, the first two-color immunofluorescence systems used a 488
nm argon laser to excite FITC and a 568 nm krypton laser to excite
TexasRed or XRITC; the dye laser at 590 nm provides better excitation,
but the major reason people moved away from 568 nm is that it is hard
to keep the 568 nm light out of the phycoerythrin channel If that's not
a problem, 150 mW at 568 would probably work. -Howard
CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
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, EMAIL
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