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Currently several companies supply Annexin V-FITC. The product quality may vary with the source. We
have used the Annexin V-FITC probe from R&D and from NeXins Research. There is a clear difference
between the two products. The Annexin V-FITC probe of NeXins is much brighter then the R&D's and
distinguishes subpopulations of cells with intermediate levels of PS exposure. These are likely early
apoptotic cells, which have not yet fragmented DNA. We have, however, not yet combined directly a
TUNEL assay with the Annexin V-assay. Indications that early apoptotic cells expose PS prior to DNA
fragmentation come from studies comparing PS exposure and nuclear morphology by flow cytometry,
light- and electron microscopy.
Chris Reutelingsperger, Ph.D.
Department of Biochemistry
University of Limburg
Maastricht, the Netherlands
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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web