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We have experienced a dramatic decrease in PE fluorescence using CD4-PE
CD1-PE and CD45RA-PE after treating the cells with Fix and Perm from
Caltag when we stained human thymocytes and a whole blood preparation of
human PBL. Other antibodies conjugated to PE show only minor flurescence
decrease. In addition the same clone of CD4-PE maintaned its
fluorescence on PBL, but not on thynocytes.
Has anybody experienced similar problems? Can you suggest a solution on
how to prevent this? So far Caltag hasn't been able to provide anithing
but simpathy...
Thanks!!!!!
Ingrid Schmid
schmid@cyto.medsch.ucla.edu
UCLA Cellular Immunology and Cytometry
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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
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