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Another technical question if you don't mind... for the FACStar users.
On a circuit card #15 there is a little knob for the 5 decade log
amplification adjust of FSC (and for SSC, FL1, FL2). Now the question is:
does it work with the FSC photodiode or just with the photomultipliers of
SSC, etc ? When I turn it full CW or CCW I cannot see any difference in FSC
signal (in log amplification of course). Maybe it is O.K., I just couldn't
find anything about it in my manuals.
The problem is I get poor resolution in FSC vs SSC dot-plot. The FSC signal
is somewhat 'widened' and there is virtually no separation of monocytes and
granulocytes, with a rather big noise (or sth that looks like random events
all over the place). I do not even mention analyzing platelets...! The same
sample ran on a friend's Coulter Elite sorter looked a LOT better (like the
best pics in the books) so I assume I had prepared the sample o.k.
Or maybe it is a peculiarity of FACStar, that poor resolution...;-)
Masses of thanx in advance,
Janek
______________________________________________
Janek Brozek e-mail: udbrozek@cyf-kr.edu.pl
http://www.cyf-kr.edu.pl/~udbrozek
2nd Dept of Internal Medicine
Allergy and Immunology Clinic
Jagiellonian University School of Medicine
Krakow, Poland
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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web