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Does anyone have experience using flow to evaluate B cell/plasma cell
populations? Especially:
-Has anyone used flow to evaluate B cell responses to specific antigens?
Can it be done or is this below the sensitivity of the technique? What
antigens are used to identify the B cells or plasma cells?
- As a first step to attempting the above, I have tried looking at human B
cells and plasma cells post "pan-B/plasma cell stimulation" with in vitro
PWM stimulation and find
- the % of cells that are B or plasma is surprisingly low (approx. 10-15%
of lymphocytes)
- I get surface staining with anti-Ig monoclonal antibodies, as well as
cytoplasmic. In fact, my % positive with surface staining is comparable to
the % I get with cytoplasmic staining. Do others see this or is this an
artifact (Fc receptor or something else?)
Many thanks,
Janine Jason
Immunology Branch,
DASTLR, NCID,CDC
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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web