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It seems that most people place the tissue in RPMI1640 with Fetal calf sera
prior to transport and processing. A sizable minority use RPMI1640 without added
sera. Time of processing does not seem to be a major concern as some people let
samples sit overnight prior to processing. Different people apparently prepare
their cell suspensions slightly differently, however everyone seems to use
fairly gentle techniques.
Thanks again for your help.
Karen Mann
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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web