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I am now in the process of writing up a chapter for the Current Protcols in
Cytometry. As it happens, I am just done with "the mitochondria".
If you want to quantify mitochondria per se you best use MitoTracker Green
(Molecular Probes, Inc. # 7514). This dye is 488 nm excitable and gives
bright (and narrow) green fluorescence. Another dye from Molecular Probes,
CMXRos (# 7512) gives red fluorescence and responds to changes in
mitochondrial membrane potential. Both dyes are retained after fixation
with aldehydes.
I do not recommend rhodamine 123, because its cell staining is so
photo-unstable; JC-1 does funny things in my hands and appears to give a lot
of red background staining outside of the mitochondria. Molecular Probes,
Inc. may be able to give you more information (Tel: 541-465-8300; FAX:
541-344-6504).
I have no idea as to how Red 613 relates to mitochondria.
Hope this helps,
Martin Poot
martin@probes.mhs.compuserve.com
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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web