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As to the question of "how much?"...if you add less of your specific moabs
in a cocktain, then also add less isotype. Obviously, you won't do this...
the antibodies are titred for a specific cell concentration, not suspension
volume. Therefore, add as much of the isotype as you do the specific anti-
sera. Staining volume is also an important issue...but that's another
discussion.
MAK.
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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web