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There is, in addition, the added complication if using directly conjugated
antibodies. For the isotype control to be valid, it would also have to match
the staining antibody with respect to its F/P ratio. And accurate
determination of F/P ratios for antibodies used in flow cytometry is a huge can
of worms.....
Alice Givan
Englert Cell Analysis Laboratory
Dartmouth Medical School
Lebanon, New Hampshire
USA
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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web