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2. Isotypic controls:
a) Can one combine two (or more) differing isotypes
(eg/ IgG1 and IgG2a) conjugated to the same fluochrome
(eg/ FITC) in one tube.
SO, I was wondering if all 5 isotypic controls needed could
be batched together in one tube???
If you put two different isotypes labeled with the same fluorochrome
in the same tube and got some nonspecific binding, then to which
isotype would you attribute the NSB signal?
Steve Micko
Emory Hospital
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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web