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>>Can someone please tell me if there is any benefit to using density gradient
>>separation on cells from a lymph node (be it a biopsy or FNA) if you are
>>already using viability gating?
>>What I saw with viability gating looked pretty straight forward, and I
>>couldn't see why any separation media would be needed.
>>I would be interested in any and all experience people have had with the two
>>techniques.
>>Thanks in advance.
>>
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In my experience, it is poor medical practice, unnecessary, time consuming,
and overly expen$ive to pass this type of specimen over a gradient.
>
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Dr. Frederic I. Preffer
preffer@helix.mgh.harvard.edu
Department of Pathology- Cox5
100 Blossom St
Massachusetts General Hospital
Boston MA 02114
(617) 726-7481 {fax x2365}
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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web