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I am planning to run a survival bioassay were then I can quantitate LIVE =
cells only using the flowcytometer.
I called Molecular Probes and they suggested "SYTOX" which stains dead =
cells only (but will not cross the membrane of live cells). Have anyone =
used this stain before? Advantages or Disadvantages?
or is there a live cell staining? Suggestions......
Osama Nabulsi
AMGEN INC.
(805)447-1594
Hi Osama,
Try using "Green Cell Tracker dye(CMFDA)" from Molecular Probes. This stains
viable cells at 1.0 uM concentrations. Staining is immediate with fluorescence
excitation at 490nm.
Good Luck,
Paul Hallberg
Retrovirology/HMJF
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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web