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>I am planning to run a survival bioassay were then I can quantitate LIVE
cells only >using the flowcytometer.
>I called Molecular Probes and they suggested "SYTOX" which stains dead cells
>only (but will not cross the membrane of live cells). Have anyone used
this stain >before? Advantages or Disadvantages?
>or is there a live cell staining? Suggestions......
Have you tried using a combination of FDA & PI? FDA will stain live cells
only and PI will stain dead cells. If you do a two color staining, you can
get an accurate count of live cells.
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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web