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Regardiing your comments on analysis of whole blood using antibodies to
GP IIbIIIa (CD41, 42a or 61)- why do you lyse the RBC's? We routinely
perform whole blood flow analysis of platelet activation (in vivo or in
vitro) without any lysis step. I would be concerned about RBC ghosts
which may have similar FALS to platelets, and I don't see any advantage
to lysing.
Vince Shankey
Loyola niv. Med. Cntr.
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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web