Requestion information on chymopapain - reply

Antony Bakke (bakkea@ohsu.EDU)
Mon, 26 Feb 96 08:59:37 PST

Any protease will remove some of the surface markers on the cell.
This is a significant problem when working with cell lines that
attach to the surface of the culture dish. We have used EDTA and
mechanical disruption to make cell suspensions instead of proteases.
Some surface antigens will be re-expressed on the cell surface
within a few hours, but the cells often clump or attach in this time
also, putting you back where you started.

Hope this helps,
Tony Bakke


Home Page Table of Contents Sponsors E-Mail Archive Web Sites

CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the Purdue University Cytometry Laboratories and distributed free of charge as an educational service to the cytometry community. If you have any comments please direct them to Dr. J. Paul Robinson, Professor & Director, PUCL, Purdue University, West Lafayette, IN 47907. Phone: (765)-494-0757; FAX(765) 494-0517; Web http://www.cyto.purdue.edu , EMAIL cdrom3@flowcyt.cyto.purdue.edu