 |
 |
 |
 |
 |
>
> I am hoping that someone can give me some (any!) information on the effect of
> chymopapain on surface markers.
Some are clipped, some are not. I would recommend incubating cell lines
and testing to see how things are affected in your own system.
>
> Another related question, does anyone know a gentle method of removing
> antibody from CD34?
Aside from chymo, cedarlane markets a sialoprotease which works well.
Dynal has a nice system with mag beads and "detachabead" anti-FAB frags
which works great if you can afford it.
Steve Gore
Johns Hopkins Oncology Center
|
|
|
|
|
|
CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web