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I would like to know the special tricks to use, if one sorts fixed human
cells for RNA / DNA / Protein extraction afterwards. I think because of
fixation (which has to be done for security reasons), there could be a
problem of RNA degradation etc... Do you add RNAse, DNAase or Protein
inhibitors to the samples ?
I know that this subject was probably already on the subject list, but I
don't have good access to the archives (from Europe it's quite slow).. so I
would still be interested in getting some hints.
thank you very much,
Matthias
______________________________________________________________
Matthias Haury _/_/_/ _/_/_/ _/_/_/
Flowcytometry - Immunology _/ _/ _/ _/ _/
Institut Pasteur Paris _/ _/_/_/ _/_/_/
Email: mhaury@pasteur.fr _/ _/ _/
Tel: 33 (1) 40 61 31 29 _/_/_/ _/ _/
Fax: 33 (1) 45 68 86 39 INSTITUT PASTEUR PARIS
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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
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