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Hi
Maybe you could use colcemid to block your cells in mitosis then do your
PI and FITC. After 12 hours or so depending on how fast your cells grow the
majority of cells in the G2/M peak will be mitotic. With adherent cell
lines, blocking with colcemid then using mitotic shake-off yields a
suspension of almost exclusivly mitotic cells.
Have fun.
Simon Monard
Aaron Diamond Center
NYC
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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web