enhanced chemiluminescence vs. flow cytometry
Alan Salkind (nepsy@umsmed.edu)
Tue, 13 Feb 1996 14:56:05 -0600
We observe relatively small changes (approximately 10%) in the mean
fluorescence intensity of an intracellular antigen by flow cytometry.
However, when we analyze cell lysates by immunoblotting and ECL the signal
is significantly increased. Is this a problem of comparing apples with
oranges? Or is ECL much more sensitive for detecting small changes in
antigen levels?
Thanks in advance for your comments and ideas.
CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web
http://www.cyto.purdue.edu
, EMAIL
cdrom3@flowcyt.cyto.purdue.edu
