AO for DNA/RNA
darzynk@nymc.edu
Mon, 22 May 1995 12:26:02 -0500
Dr. Watkins inquired about the simple AO procedure of cell staining
for DNA and RNA. The methodology of use of AO for differential
staining of RNA vs. DNA is presented in detail in Vol 41 of Methods
in Cell Biology (1994; Chapter 26, pp 401-420). Once the solutions
are made the procedure is rapid - it takes only 2 minutes from taking
the cell suspension directly from the cell culture to measuring its
fluorescence. The most essential requirement is the proper
concentration of AO in the staining solution.
Zbigniew Darzynkiewicz
CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web
http://www.cyto.purdue.edu
, EMAIL
cdrom3@flowcyt.cyto.purdue.edu
