Re: TdT control

sperfetto@hiv.hjf.org
Mon, 22 May 1995 08:55:31 -0400

It is my experience that the reagents in the Tdt kit have a limit shelf life
that can lead to deviations in experimental results...Beyond this, how would you
interpret an assay without positive and negative control? Mouse thymocytes
treated with dexamethasone (-10^7M) or irradiated serve as good +controls; if
using human cells, treat cultures with 100uM pervanadate for 48hrs and use these
as +controls...GOOD LUCK!
_______________________________________________________________________________
Subject: TdT control
From: SPERFETTO
Date: 5/19/95 4:18 PM

Does one need to run a positive (and a negative?) control with TdT? We used
to have a + and a - control for slide based TdT. Somehow I donot think we
need those controls, now that we have switched to flow TdT.
Bakul I. Dalal MD FRCPC FCAP FASCP
Division of Hematopathology, LSP1
Vancouver Hospital and Health Sciences Center,
910 West 10th Avenue,
Vancouver, BC, CANADA

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